First report on the production of phytotoxic metabolites by Mycoleptodiscus indicus under optimized conditions of submerged fermentation

• Published in: Environmental technology Vol. 43; no. 10; pp. 1458 - 1470
• Main Authors: Portela, Valéria Ortaça, Moro, Anderson, Santana, Natielo Almeida, Baldoni, Daiana Bortoluzzi, de Castro, Isac Aires, Antoniolli, Zaida Inês, Dalcol, Ionara Irion, Seminoti Jacques, Rodrigo Josemar
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 29-04-2022; Taylor & Francis Ltd
• Subjects: Ascomycota - metabolism; bioherbicide; Conyza spp; Culture media; Culture Media - metabolism; Culture Media - pharmacology; Design optimization; Fermentation; fungal metabolites; Fungi; Germination; Glucose; Herbicides; Herbicides - toxicity; Leaves; Lesions; Liquid fermentation; Low cost; Metabolites; Mycoleptodiscus; Plant Weeds - metabolism; Plants; Seedlings; Signs and symptoms; Weeds; Yeasts; bioherbicide; weeds; fungal metabolites; Liquid fermentation; Conyza spp
• ISSN: 0959-3330; 1479-487X
• DOI: 10.1080/09593330.2020.1836030

An alternative to controlling weeds resistant to conventional herbicides is the isolation of new active principles. Fungi can produce phytotoxic metabolites that may be used in the development of new herbicides. The objectives of this study were: (1) isolate, select, and identify a fungus producer of phytotoxic metabolites and (2) optimize the culture conditions of this fungus in a low-cost culture medium, with the aim of increasing the phytotoxic effects of their metabolites in weeds and commercial plants. Fungi were isolated from the leaves of Conyza sp. with disease symptoms and selected according to the production of phytotoxic metabolites in solid and submerged fermentation in a low-cost culture medium. A Plackett-Burman Design and Central Composite Rotational Design were used to optimize the conditions of temperature, agitation, pH, and concentrations of glucose and yeast extract in submerged fermentation. The phytotoxic metabolites produced under optimal conditions were tested on 10 commercial plants and weeds that are difficult to control. Of the nine fungi isolated, Mycoleptodiscus indicus UFSM54 produced higher leaf lesions. The production of phytotoxic metabolites was optimized when the fungus was cultivated at 35°C, 50 rpm, and 1.5 g L
−1
of glucose in submerged fermentation. The metabolites of M. indicus caused severe phytotoxic effects on germination and seedling growth, and enhanced lesion development on detached plant leaves. The present study is the first to report on the production of phytotoxic metabolites by M. indicus, a potential producer of bioherbicides.