Structural variations on Salmonella biofilm by exposition to river water

• Published in: International journal of environmental health research Vol. 32; no. 7; pp. 1626 - 1643
• Main Authors: MB, Contreras-Soto, JA, Medrano-Félix, JA, Sañudo-Barajas, R, Vélez-de la Rocha, JR, Ibarra-Rodríguez, J, Martínez-Urtaza, C, Chaidez, N, Castro-del Campo
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03-07-2022; Taylor & Francis LLC
• Subjects: biofilm composition; Biofilms; Chemical composition; Galactose; Mannose; morphotype; non-host environment; Pellicle; Physical characteristics; Ribose; river water exposition; Rivers; Salmonella; Serotypes; Surface water; Salmonella; morphotype; non-host environment; river water exposition; biofilm composition
• ISSN: 0960-3123; 1369-1619
• DOI: 10.1080/09603123.2021.1901863

Biofilm formation, as adapting strategies, is the result of stressful conditions that Salmonella faces in hostile environments like surface water. We evaluated river water effect on Salmonella biofilm formation ability in terms of physical, morphological characteristics and chemical composition. A new morphotype SPAM (soft, pink and mucoid) was detected in Oranienburg strains S-76 and S-347 (environmental and clinical isolate). Oranienburg serotypes showed very marked behavior in adherence, pellicle liquid-air and resistance, being Oranienburg S-76 the strongest biofilm producer. All strains when exposed to river water presented an overlapping mucoid layer in the morphotype and increased their motility except Oranienburg S-347. The most motile was Typhimurium (control) and the least Infantis S-304 (clinical isolate). Mannose, glucose, galactose and ribose were the main biofilm sugar components; type and concentration of sugar suggest a morphotype/serotype dependent pattern. Strong morphotypes expressed in this study may be an effective protective strategy for Salmonella in hostile environments.