Terminaliamide, a new ceramide and other phytoconstituents from the roots of Terminalia mantaly H. Perrier and their biological activities

• Published in: Natural product research Vol. 35; no. 8; pp. 1313 - 1322
• Main Authors: Mbosso Teinkela, Jean Emmanuel, Siwe Noundou, Xavier, Fannang, Simone, Mbem Song, Achille, Assob Nguedia, Jules Clément, Hoppe, Heinrich C., Krause, Rui Werner Maçedo
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-04-2021; Taylor & Francis Ltd
• Subjects: Anti-Infective Agents - pharmacology; Antibacterial activity; Antimalarials - chemistry; Antimalarials - isolation & purification; Antimalarials - pharmacology; antimicrobial; antiplasmodial; Antiprotozoal agents; antitrypanosomal; Bacteria - drug effects; Carbon-13 Magnetic Resonance Spectroscopy; Cell Survival - drug effects; Ceramide; Ceramides - chemistry; Ceramides - isolation & purification; Ceramides - pharmacology; Cytotoxicity; HeLa Cells; Hexanes; Humans; Magnetic resonance spectroscopy; Mass spectrometry; Mass spectroscopy; Microbial Sensitivity Tests; Minimum inhibitory concentration; NMR; NMR spectroscopy; Nuclear magnetic resonance; Phytochemicals - chemistry; Phytochemicals - isolation & purification; Phytochemicals - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Roots - chemistry; Plasmodium falciparum - drug effects; Plasmodium falciparum - growth & development; Proton Magnetic Resonance Spectroscopy; Protozoa; Roots; Terminalia - chemistry; Terminalia mantaly; Terminaliamide; Toxicity; Trypanosoma brucei brucei - drug effects; Two dimensional analysis; Terminalia mantaly; antitrypanosomal; antimicrobial; Terminaliamide; antiplasmodial; cytotoxicity
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2019.1647425

Terminaliamide (1), a new ceramide was isolated from the roots of Terminalia mantaly H. Perrier (Combretaceae) along with 4 known compounds (2-5). The structures of the compounds were elucidated using 1D and 2D NMR spectroscopy analysis and mass spectrometry. Compound 1 exhibited moderated antibacterial activity towards Staphylococcus aureus with MIC value of 62.5 μg/mL. The crude MeOH extract (TMr) highly reduced Plasmodium falciparum growth with an IC
50
value of 10.11 μg/mL, while hexane fraction (F1) highly reduced Trypanosoma brucei brucei growth with an IC
50
value of 5.60 µg/mL. All tested samples presented little or no in vitro cytotoxicity on HeLa cell line. The present work confirms that T. mantaly is medicinally important and may be used effectively as an antimicrobial, an antiplasmodial and an antitrypanosomial with promising therapeutic index.