Molecular machinery of starch digestion and glucose absorption along the midgut of Musca domestica

• Published in: Journal of insect physiology Vol. 109; pp. 11 - 20
• Main Authors: Pimentel, André C., Barroso, Ignacio G., Ferreira, Jéssica M.J., Dias, Renata O., Ferreira, Clélia, Terra, Walter R.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-08-2018
• Subjects: Amylase; Amyrel; Maltase; Sugar transporters; Transporter up-regulation; α-Glucosidase; Maltase; Amylase; α-Glucosidase; Transporter up-regulation; Sugar transporters; Amyrel
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2018.05.009

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•Triplicate RNA-seq analyses were prepared from midgut sections and carcass.•Enzymes and sugar transporters (SP) were searched in the reference genome.•The expression of two amylases, three maltases, and 9 SPs were determined.•MdSP1 (apical), MdSP7 (basal), and MdSP9 (apical) are up-regulated by starch.•A model is proposed for the stepwise digestion and absorption of starch/glycogen.
Until now there is no molecular model of starch digestion and absorption of the resulting glucose molecules along the larval midgut of Musca domestica. For addressing to this, we used RNA-seq analyses from seven sections of the midgut and carcass to evaluate the expression level of the genes coding for amylases, maltases and sugar transporters (SP). An amylase related protein (Amyrel) and two amylase sequences, one soluble and one with a predicted GPI-anchor, were identified. Three highly expressed maltase genes were correlated with biochemically characterized maltases: one soluble, other glycocalyx-associated, and another membrane-bound. SPs were checked as being apical or basal by proteomics of microvillar preparations and those up-regulated by starch were identified by real time PCR. From the 9 SP sequences with high expression in midgut, two are putative sugar sensors (MdSP4 and MdSP5), one is probably a trehalose transporter (MdSP8), whereas MdSP1-3, MdSP6, and MdSP9 are supposed to transport glucose into cells, and MdSP7 from cells to hemolymph. MdSP1, MdSP7, and MdSP9 are up-regulated by starch. Based on the data, starch is at first digested by amylase and maltases at anterior midgut, with the resulting glucose units absorbed at middle midgut. At this region, low pH, lysozyme, and cathepsin D open the ingested bacteria and fungi cells, freeing sugars and glycogen. This and the remaining dietary starch are digested by amylase and maltases at the end of middle midgut and up to the middle part of the posterior midgut, with resulting sugars being absorbed along the posterior midgut.