Variation and Intraspecific Relationships in Indian Wild Musa balbisiana (BB) Population as Evidenced by Random Amplified Polymorphic DNA

• Published in: Genetic resources and crop evolution Vol. 53; no. 2; pp. 349 - 355
• Main Authors: Uma, S, Siva, S.A, Saraswathi, M.S, Manickavasagam, M, Durai, P, Selvarajan, R, Sathiamoorthy, S
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01-03-2006
• Subjects: Amplification; bananas; Deoxyribonucleic acid; DNA; Fragments; fruit crops; genetic polymorphism; genetic variation; geographical variation; Intraspecific relationships; Musa; Musa balbisiana; plant morphology; plant taxonomy; Polymerase chain reaction; Polymorphism; Primers; Random amplified polymorphic DNA; random amplified polymorphic DNA technique; wild plants; wild relatives; India
• ISSN: 0925-9864; 1573-5109
• DOI: 10.1007/s10722-004-0576-y

Sixteen collections of the wild Musa species, Musa balbisiana Colla collected from different regions of India were studied for their intraspecific relationships using random amplified polymorphic DNA (RAPD) markers. Out of 80 primers screened, 34 primers produced reproducible bands and four primers among them showing polymorphic bands were used. In all, 43 DNA fragments were amplified averaging 10.75 per primer. Of these, 31 amplified fragments showed polymorphism (averaging of 7.75 per primer). The extent of polymorphism (74.6%) has indicated the existence of considerable variation at the DNA level within the species. The 16 accessions were clustered into four as against seven clusters obtained through morphotaxonomic characterization. The inter relationships based on geographical origin in comparison with molecular characterization have been discussed.