Evaluation of total phenols content, anti-DPPH activity and the content of selected antioxidants in the honeybee drone brood homogenate

• Published in: Food chemistry Vol. 368; p. 130745
• Main Authors: Sawczuk, Róża, Karpinska, Joanna, Filipowska, Diana, Bajguz, Andrzej, Hryniewicka, Marta
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 30-01-2022
• Subjects: All-trans-retinol; Antioxidant properties; Ascorbic acid; Coenzyme Q10; Drone brood homogenate; α-Tocopherol; α-Tocopherol; Antioxidant properties; Drone brood homogenate; Ascorbic acid; Coenzyme Q10; All-trans-retinol
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2021.130745

•Drone brood homogenate (DBH) was tested for antioxidant properties.•Vitamin C, coenzyme Q10, α-tocopherol, and retinol in DBH samples was assessed.•Condition of bees and apiary environment affect the amount of lipophilic antioxidants.•DBH is a rich source of coenzyme Q10, α-tocopherol, vitamin C, and phenols.
The subject of the present research is the evaluation of health-promoting properties caused by the presence of some vitamins as well as the antioxidative potential of the honeybee drone brood homogenate (DBH). The study used 139 homogenate samples obtained from various apiaries and collected over 3 years, three times during each beekeeping season. Samples differed in terms of varroa infestation, stage of brood development, location of the apiary, and the degree of environmental contamination. The content of ascorbic acid, α-tocopherol, all-trans-retinol, and coenzyme Q10 in the tested samples was determined through the application of HPLC/DAD/UV and LC/QQQ/MS methods. The antioxidant potential of samples was assessed using the Folin-Ciocalteu and DPPH methods.