In situ study of ligand–receptor interaction by total internal reflection ellipsometry

• Published in: Thin solid films Vol. 571; pp. 744 - 748
• Main Authors: Balevicius, Z., Baleviciute, I., Tumenas, S., Tamosaitis, L., Stirke, A., Makaraviciute, A., Ramanaviciene, A., Ramanavicius, A.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 28-11-2014; Elsevier
• Subjects: Condensed matter: electronic structure, electrical, magnetic, and optical properties; Dynamic tests; Ellipsometry; Exact sciences and technology; Granulocyte colony stimulating factor (GCSF); In situ spectroscopic ellipsometry; Instruments, apparatus, components and techniques common to several branches of physics and astronomy; Ligand-receptor interaction; Nonlinear dynamics; Optical instruments, equipment and techniques; Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation; Optical properties of specific thin films; Physics; Polarimeters and ellipsometers; Receptors; Reflection; Spectroscopic analysis; Spectroscopy; SPR enchanced ellipsometry; Tires; Total internal reflection ellipsometry; Granulocyte colony stimulating factor (GCSF); SPR enchanced ellipsometry; In situ spectroscopic ellipsometry; Ligand-receptor interaction; Total internal reflection ellipsometry; Spectroscopic ellipsometry; Data acquisition; Refractive index; Optical properties; Monolayers; Ligands; Dispersions
• ISSN: 0040-6090; 1879-2731
• DOI: 10.1016/j.tsf.2013.10.090

Spectroscopic total internal reflection ellipsometry (TIRE) in its dynamic data acquisition mode has been applied for the in situ study of the interactions of monomeric (mGCSF) and dimeric (dGCSF) granulocyte colony stimulating factor and its receptor (GCSF-R) which was immobilized on a thin gold layer. Regression results have shown that the account for signal non-linearity due to analysis of spectroscopic dynamic TIRE data has allowed us to detect small differences in association and dissociation times through the precise determination of the refractive index dispersion. Different binding models were applied in the study of mGCSF and dGCSF interaction with a monolayer of its receptor GCSF-R due to their distinctive changes of refractive index over time.
•Spectroscopic TIRE in dynamic acquisition mode was applied for protein study.•Elimination of signal non-linearity gives more precise values of refractive index.•Different binding models were applied for mGCSF and dGCSF interaction with GCSF-R.