Dynamic heterogeneity and non-Gaussian statistics for ganglioside GM1s and acetylcholine receptors on live cell membrane

• Published in: Molecular biology of the cell Vol. 31; no. 13; pp. mbcE19080473 - 1391
• Main Authors: He, Wei, Su, Yun, Peng, H Benjamin, Tong, Penger
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 15-06-2020
• ISSN: 1059-1524; 1939-4586
• DOI: 10.1091/mbc.E19-08-0473

We have carried out a comparative study of the lateral motion of ganglioside GM1, which is a glycosphingolipid residing on the outer leaflet of the plasma membrane, and acetylcholine receptor (AChR), which is a well-characterized ion channel. Both the lipid molecules and transmembrane proteins reside on the plasma membrane of live Xenopus muscle cells. From a thorough analysis of a large volume of individual molecular trajectories obtained from more than 300 live cells over a wide range of sampling rates and long durations, we find that the GM1s and AChRs share the same dynamic heterogeneity and non-Gaussian statistics. Our measurements with the ATP-depleted cells reveal that the diffusion dynamics of the GM1s and AChRs is uniformly affected by the intracellular ATP level of the living muscle cells, further demonstrating that membrane diffusion is strongly coupled to the dynamics of the underlying cortical actin network, as predicted by the dynamic picket-fence model [W. He et al., Nature Communications, 7:11701 (2016)].