Anti-diabetic functional food with wasted litchi seed and standard of quality control

• Published in: Applied biological chemistry Vol. 60; no. 2; pp. 197 - 204
• Main Authors: Choi, Se-Ah, Lee, Ji Eun, Kyung, Min Ji, Youn, Ju Hee, Oh, Jeong Bin, Whang, Wan Kyunn
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-04-2017; Springer Nature B.V; 한국응용생명화학회
• Subjects: Acarbose; Alternative medicine; Applied Microbiology; Bioactive compounds; Biological activity; Biological Techniques; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Chronic illnesses; Diabetes; Diabetes mellitus; Economic impact; Food industry; Food processing industry; Functional foods & nutraceuticals; Glucosidase; Liquid chromatography; Mass spectrometry; Mass spectroscopy; NMR; Nuclear magnetic resonance; Protein-tyrosine-phosphatase; Quadrupoles; Quality control; Quality of life; Scientific imaging; Spectroscopy; Tyrosine; Ursolic acid; α-Glucosidase; 농학; Anti-diabetic agent; Bioactivity-guided isolation; Protein tyrosine phosphatase 1B inhibitory assay; α-Glucosidase inhibitory assay; Litchi seed; Quality control standard
• ISSN: 2468-0834; 2468-0842
• DOI: 10.1007/s13765-017-0269-9

The prevalence of diabetes has become a huge health burden in parts of quality of life and economic cost for overcoming this chronic disease. We followed a bioactivity-guided isolation using α-glucosidase inhibitory assay, four major compounds were isolated, and their structures were elucidated using nuclear magnetic resonance, mass spectrometry, and ultra-performance liquid chromatography quadrupole time of flight mass spectrometry in litchi seed. As a result, the IC
50
of α-glucosidase inhibitory assay of the crude extract, sugar-removed layer, pavetannin B2, procyanidin A2, and acarbose was 0.691 μg/mL, 3.686 μg/mL, 0.04 μM, 0.08 μM, and 55.845 μg/mL, respectively. With those compounds, we examined the protein tyrosine phosphatase 1B inhibitory activity. And the IC
50
of pavetannin B2, procyanidin A2, and ursolic acid was 450.295, 338.257, and 19.686 μM, respectively. Contents analysis method for bioactive compounds, which can be used in manufacturing for extract preparations, was established. The findings of this study, litchi seed can be a cost-effective medicinal food in terms of recyclable resources in the litchi food industry and as a natural alternative medicine against type-2 diabetes.