The impact of histones linked to sperm chromatin on embryo development and ART outcome

• Published in: Andrology (Oxford) Vol. 6; no. 3; pp. 436 - 445
• Main Authors: Fournier, C., Labrune, E., Lornage, J., Soignon, G., Giscard d'Estaing, S., Guérin, J.‐F., Benchaib, M.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01-05-2018
• Subjects: Adult; ART outcome; Chromatin - metabolism; Chromatin - pathology; Cohort Studies; DFI; embryo development; Embryonic Development; Embryos; Female; histone; Histones - metabolism; Humans; Male; Pregnancy; Pregnancy Rate; Prospective Studies; Protamines - metabolism; Reproductive Techniques, Assisted; Sperm; sperm chromatin; Spermatozoa - metabolism; Spermatozoa - pathology; ART outcome; embryo development; histone; sperm chromatin; DFI
• ISSN: 2047-2919; 2047-2927
• DOI: 10.1111/andr.12478

Summary
The purpose of this study was to investigate the relationship between the proportion of sperm chromatin linked to remaining histone and assisted reproductive technology (ART) outcome. A prospective cohort study was performed on couples undergoing ART process at the Department of Reproduction Medicine (HFME, Bron, France). The histone‐to‐protamine ratio (HPR) was measured using the method described by Wykes & Krawetz (2003) J Biol Chem 278, 29471. The correlations with sperm DFI, blastocyst formation, pregnancy rate, and delivery rate were investigated. A total of 291 ART cycles were included (42 c‐IVF and 249 ICSI procedures): 3870 oocytes were punctured and 2211 embryos were obtained, among which 507 were transferred and 336 frozen. The mean HPR was 18.9%. A significant negative correlation was found between HPR and DFI (r = −0.12, p < 0.05). Regarding the type of ART procedure (c‐IVF or ICSI), the same kind of relationship between HPR and ART parameters was observed. Regardless of the type of ART procedure used, when the HPR was within the range [6%; 26%], the blastocyst formation rate was higher: 87.8% vs. 71.2% (HPR<6%; p < 0.01) and 74.6% (HPR >26%; p < 0.01). The highest delivery rate (DR; 24.5%) was obtained for HPR within the range [6%; 26%]; DR was 21.9% for HPR<6% and 18.3% for HPR>26%; however, the differences were not statistically significant. The procedure described in this study seems to be a reliable evaluation of the HPR. The HPR parameter seems to be correlated to embryonic development up to the blastocyst stage, but its involvement in clinical pregnancy/delivery could not be confirmed. HPR should be further investigated for confirming the relationship with blastocyst formation. After this, the next step will be to investigate the etiologies of HPR alterations for improving the sperm nucleus quality for increasing the chance of pregnancy.