Tightening the retinal glia limitans attenuates neuroinflammation after optic nerve injury

• Published in: Glia Vol. 68; no. 12; pp. 2643 - 2660
• Main Authors: Lefevere, Evy, Salinas‐Navarro, Manuel, Andries, Lien, Noterdaeme, Lut, Etienne, Isabelle, Van Wonterghem, Elien, Vinckier, Stefan, Davis, Benjamin M., Van Bergen, Tine, Van Hove, Inge, Movahedi, Kiavash, Vandenbroucke, Roosmarijn E., Moons, Lieve, De Groef, Lies
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-12-2020; Wiley Subscription Services, Inc
• Subjects: Astrocytes; Central nervous system; Cytokines; glia limitans; Immunomodulation; Infiltration; Inflammation; Injury prevention; Interleukin 1; Leukocytes; Matrix metalloproteinase; Matrix metalloproteinases; matrix metalloproteinase‐3; Metalloproteinase; Neurodegeneration; neuroinflammation; Neuronal-glial interactions; Neuroprotection; Optic nerve; optic nerve crush; Retina; Structure-function relationships; Tightening; neurodegeneration; optic nerve crush; matrix metalloproteinase-3; neuroinflammation; glia limitans
• ISSN: 0894-1491; 1098-1136
• DOI: 10.1002/glia.23875

Increasing evidence suggests that functional impairments at the level of the neurovascular unit (NVU) underlie many neurodegenerative and neuroinflammatory diseases. While being part of the NVU, astrocytes have been largely overlooked in this context and only recently, tightening of the glia limitans has been put forward as an important neuroprotective response to limit these injurious processes. In this study, using the retina as a central nervous system (CNS) model organ, we investigated the structure and function of the glia limitans, and reveal that the blood–retina barrier and glia limitans function as a coordinated double barrier to limit infiltration of leukocytes and immune molecules. We provide in vitro and in vivo evidence for a protective response at the NVU upon CNS injury, which evokes inflammation‐induced glia limitans tightening. Matrix metalloproteinase‐3 (MMP‐3) was found to be a crucial regulator of this process, thereby revealing its beneficial and immunomodulatory role in the CNS. in vivo experiments in which MMP‐3 activity was deleted via genetic and pharmacological approaches, combined with a comprehensive study of tight junction molecules, glial end feet markers, myeloid cell infiltration, cytokine expression and neurodegeneration, show that MMP‐3 attenuates neuroinflammation and neurodegeneration by tightening the glia limitans, thereby pointing to a prominent role of MMP‐3 in preserving the integrity of the NVU upon injury. Finally, we gathered promising evidence to suggest that IL1b, which is also regulated by MMP‐3, is at least one of the molecular messengers that induces glia limitans tightening in the injured CNS.
Main Points
In the neurovascular unit, the blood–retinal barrier and glia limitans function as a coordinated double barrier to limit infiltration of leukocytes.
MMP‐3 contributes to preserving the integrity of the retinal neurovascular unit upon optic nerve crush by attenuating neuroinflammation and neurodegeneration.