Application of a dual target PCR-high resolution melting (HRM) method for rapid nontuberculous mycobacteria identification

Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate...

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Bibliographic Details
Published in:	Journal of microbiological methods Vol. 132; pp. 1 - 3
Main Authors:	Chen, Jonathan HK, Cheng, Vincent CC, She, Kevin KK, Yam, Wing-Cheong, Yuen, Kwok-Yung
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-01-2017
Subjects:	Bacterial Proteins - genetics Chaperonin 60 - genetics Cost-Benefit Analysis DNA, Bacterial - isolation & purification Genes, Bacterial High-resolution melting Nontuberculous mycobacteria Nontuberculous Mycobacteria - classification Nontuberculous Mycobacteria - isolation & purification Real-Time Polymerase Chain Reaction RNA, Ribosomal, 16S - isolation & purification High-resolution melting Nontuberculous mycobacteria
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Summary:	Species differentiation of nontuberculous mycobacteria (NTM) has long been a difficult task in clinical laboratories. This study demonstrated and evaluated a simple and cost-effective method using the real-time PCR with high-resolution melting (PCR-HRM) analysis technique, which could differentiate at least 14 different medically related NTM.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2016.11.004