Real-time PCR detection of Brucella spp. DNA in lesions and viscera of bovine carcasses

• Published in: Journal of microbiological methods Vol. 104; pp. 87 - 91
• Main Authors: Sola, Marília Cristina, da Veiga Jardim, Eurione A.G., de Freitas, Marcius Ribeiro, de Mesquita, Albenones José
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-09-2014
• Subjects: Animals; Assessment of carcasses and viscera; Brucella; Brucella - genetics; Brucella - isolation & purification; Brucellosis; Brucellosis - diagnosis; Brucellosis - microbiology; Brucellosis - veterinary; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - microbiology; DNA, Bacterial - genetics; FTA® Elute card; Meat - microbiology; Real-Time Polymerase Chain Reaction - methods; Sensitivity and Specificity; Viscera - microbiology; Brucellosis; FTA® Elute card; Assessment of carcasses and viscera
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2014.06.015

This study reports a real-time PCR assay for the detection of Brucella spp. associated with the FTA® Elute method in lesions observed during sanitary inspections in beef slaughter. Of the total 276 samples, 78 (28.3%) tested positive and 198 (71.7%) negative for Brucella spp. The real-time PCR technique associated with the FTA® Elute method proved to be an important tool for the diagnosis, judgment about and disposal of carcasses and viscera of slaughtered animals.
•Our aim was to support the detection of Brucella spp. from lesions in cattle.•We associated the Real-time PCR assay with FTA® Elute cards for diagnosis.•It can be considered as an important diagnostic tool for use in slaughterhouses.