Evaluation of an immunochromatographic assay for direct identification of thermostable direct hemolysin-producing Vibrio parahaemolyticus colonies on selective agar plates

• Published in: Journal of microbiological methods Vol. 119; pp. 4 - 6
• Main Authors: Kawatsu, Kentaro, Sakata, Junko, Yonekita, Taro, Kumeda, Yuko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-12-2015
• Subjects: Bacterial Toxins - genetics; Bacterial Toxins - metabolism; Diarrhea; Food-borne pathogen; Hemolysin Proteins - genetics; Hemolysin Proteins - metabolism; Humans; Immunochromatographic assay; Immunochromatography - methods; TDH-producing Vibrio parahaemolyticus; Vibrio Infections - microbiology; Vibrio parahaemolyticus; Vibrio parahaemolyticus - genetics; Vibrio parahaemolyticus - isolation & purification; Vibrio parahaemolyticus - metabolism; Diarrhea; Food-borne pathogen; TDH-producing Vibrio parahaemolyticus; Immunochromatographic assay
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2015.09.020

We evaluated the utility of an immunochromatographic assay (NH IC TDH) in identifying thermostable direct hemolysin (TDH)-producing Vibrio parahaemolyticus colonies on selective agar plates. The sensitivity of the NH IC TDH assay was 100% (189 samples) and its specificity was 100% (41 samples) compared with the presence of tdh.
•An immunochromatographic assay for the identification of TDH-producing V. parahaemolyticus•The assay identifies the pathogen directly from a single agar colony.•The assay is 100% accurate compared with the presence of tdh.