Dielectric characterization of hepatocytes in suspension and embedded into two different polymeric scaffolds

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 102; pp. 700 - 707
• Main Authors: Massimi, M., Stampella, A., Devirgiliis, L. Conti, Rizzitelli, G., Barbetta, A., Dentini, M., Cametti, C.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-02-2013
• Subjects: cell growth; cell viability; colloids; dielectric properties; Dielectric Spectroscopy; gelatin; Hep G2 Cells; Hepatocytes; Hepatocytes - cytology; Hepatocytes - drug effects; Hepatocytes - metabolism; Humans; liver; nuclear membrane; Polymeric scaffolds; Polymers - adverse effects; Polymers - chemistry; qualitative analysis; tissue engineering; Tissue Scaffolds - adverse effects; Tissue Scaffolds - chemistry; Hepatocytes; Dielectric spectroscopy; Polymeric scaffolds
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2012.09.015

► Polymeric scaffolds with well-controlled interconnected pores, play a pivotal role in tissue engineering. ► The understanding of the principles of scaffolding is far from satisfactory ► Dielectric relaxation spectroscopy is used to monitor hepatocyte growth in two different polymeric scaffolds ► Biocompatibility of gelatine and alginate scaffolds is discussed ► The performance of alginate scaffold in comparison with gelatine scaffold was investigated. [Display omitted]
The dielectric and conductometric properties of hepatocytes in two different environments (in aqueous suspension and embedded into polymeric scaffolds) have been investigated in the frequency range from 1kHz to 2GHz, where the interfacial electrical polarization gives rise to marked dielectric relaxation effects. We analyzed the dielectric behavior of hepatocytes in complete medium aqueous suspensions in the light of effective medium approximation for heterogeneous systems and hepatocytes cultured into two different highly porous and interconnected polymeric structures. In the former case, we have evaluated the passive electrical parameters associated with both the plasmatic and nuclear membrane, finding a general agreement with the values reported elsewhere, based on a partially different analysis of the experimental spectra. In the latter case, we have evaluated the cell growth into two different polymeric scaffolds made of alginate and gelatin with a similar pore distribution and similar inter-connectivity. Based on a qualitative analysis of the dielectric spectra, we were able to provide evidence that alginate scaffolds allow an overall survival of cells better than gelatin scaffold can do. These indications, confirmed by biological tests on cell viability, suggest that hepatocytes embedded in alginate scaffolds are able to perform liver specific functions even over on extended period of time.