Glycoproteomic measurement of site-specific polysialylation

• Published in: Analytical biochemistry Vol. 596; p. 113625
• Main Authors: Pelingon, Ruby, Pegg, Cassandra L., Zacchi, Lucia F., Phung, Toan K., Howard, Christopher B., Xu, Ping, Hardy, Matthew P., Owczarek, Catherine M., Schulz, Benjamin L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-05-2020
• Subjects: Glycoproteins - analysis; Glycoproteins - metabolism; Glycoproteomics; Humans; Mass Spectrometry; N-linked glycosylation; Polysaccharides - metabolism; Polysialylation; Proteomics; Sialic acid; Sialic Acids - analysis; Sialic Acids - metabolism; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Polysialylation; Sialic acid; Glycoproteomics; N-linked glycosylation; Mass spectrometry
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2020.113625

Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans. Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer. Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics. PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function. However, PSA is difficult to analyse by mass spectrometry (MS) due to its high negative charge and size. Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy. Here, we developed a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins. This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection. PSA-modified glycopeptides are not detectable by LC-ESI-MS/MS, but become detectable after desialylation, allowing measurement of site-specific PSA occupancy. This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.
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•Polysialic acid is a key post-translational modification of some glycoproteins.•Standard glycoproteomic mass spectrometry methods cannot measure polysiaic acid.•Desialylation allows measurement of site-specific polysialic acid occupancy.