Crystallization and preliminary X-ray studies of the basic lectin from winged bean (Psophocarpus tetragonolobus)

• Published in: Journal of molecular biology Vol. 229; no. 2; p. 558
• Main Authors: Sankaranarayanan, R, Puri, K D, Ganesh, V, Banerjee, R, Surolia, A, Vijayan, M
• Format: Journal Article
• Language: English
• Published: England 20-01-1993
• Subjects: Crystallization; Fabaceae; Lectins - chemistry; Plant Lectins; Plants, Medicinal; X-Ray Diffraction
• ISSN: 0022-2836
• DOI: 10.1006/jmbi.1993.1056

The basic lectin from winged bean (Psophocarpus tetragonolobus) could be crystallized using polyethyleneglycol (PEG) 4000 (I), PEG 8000 (II) and 2-methylpentane-2,4-diol (MPD) (III) as precipitants. Crystal forms I and II grew in the presence of methyl-alpha-D-galactopyranoside or N-acetylgalactosamine while III grew in the absence of sugar. The three forms have the same space group (P2(1)2(1)2) and similar unit cell dimensions with two dimeric molecules in the asymmetric unit. The unit cell dimensions are a = 156.8 A, b = 89.0 A, c = 73.3 A for I, a = 155.5 A, b = 92.3 A, c = 72.5 A for II and a = 148.3 A, b = 90.7 A, c = 73.8 A for III. The crystals, particularly those grown using PEG 8000, are suitable for high resolution X-ray analysis, which is in progress.