Differentiation of cattle species in beef by PCR-RFLP of mitochondrial and satellite DNA

• Published in: Meat science Vol. 60; no. 4; pp. 365 - 369
• Main Authors: Verkaar, E.L.C., Nijman, I.J., Boutaga, K., Lenstra, J.A.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-04-2002; Elsevier
• Subjects: Beef; Biological and medical sciences; Bovini; Food industries; Fundamental and applied biological sciences. Psychology; Meat and meat product industries; Mitochondrial DNA; PCR-RFLP; Satellite DNA; Species identification; Satellite DNA; Bovini; Mitochondrial DNA; PCR-RFLP; Beef; Species identification; Bovine; Meat product; Hybrid; Identification; Species specificity; Polymerase chain reaction; Vertebrata; Meat animals; Mammalia; Discrimination; Restriction fragment length polymorphism; Control method; Artiodactyla; Molecular biology; Ungulata
• ISSN: 0309-1740; 1873-4138
• DOI: 10.1016/S0309-1740(01)00144-9

Methods currently used for the identification of the species origin of meat or tissue samples have not been validated for other bovine species than taurine cattle or water buffalo. These methods also do not discriminate between the different bovine species that are used as source of beef. Here, we describe two complementary methods for detection and differentiation of bovine species, which are based on mutations in mitochondrial DNA and centromeric satellite DNA, respectively. The analysis of satellite DNA is especially relevant for the identification of animals that are of hybrid origin.