Evidence for the Presence of cAMP, cAMP Receptor and Transcription Termination Factor Rho in Different Gram-negative Bacteria

• Published in: Journal of general microbiology Vol. 131; no. 11; pp. 2953 - 2960
• Main Authors: BIVILLE, FRANCIS, GUISO, NICOLE
• Format: Journal Article
• Language: English
• Published: London Soc General Microbiol 01-11-1985; New York, NY Cambridge University Press
• Subjects: Bacteriology; Biological and medical sciences; Cyclic AMP - biosynthesis; Cyclic AMP - genetics; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Gram-Negative Bacteria - genetics; Growth, nutrition, cell differenciation; Microbiology; Radioimmunoassay; Receptors, Cyclic AMP - analysis; Receptors, Cyclic AMP - genetics; Rho Factor - analysis; Rho Factor - genetics; Transcription Factors - genetics; Transcription termination; Regulation(control); Transcription; Escherichia coli; Cyclic AMP; Bacteria; Receiver; Biosynthesis; Ratio; Escherichieae; Enterobacteriaceae; Immunological method
• ISSN: 0022-1287; 1350-0872; 1465-2080
• DOI: 10.1099/00221287-131-11-2953

Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris, Cedex 15, France
ABSTRACT
Summary: Cyclic AMP has been shown to be present in 12 different Gram-negative bacteria and the regulation of its concentration, as a function of growth conditions, is similar to that described for Escherichia coli K12. Antibodies raised against catabolite activator protein (CAP) and Rho protein of E. coli K12 were used to check for the occurrence of cross-reactive antigens. Using radioimmunological assays, immunoblotting techniques and biochemical criteria we showed a wide distribution of CAP and Rho, structurally and functionally closely related to the corresponding E. coli K12 proteins. These results suggest that transcription is similarly regulated by these factors in Gram-negative bacteria.