Antioxidant activities of phlorotannins purified from Ecklonia cava on free radical scavenging using ESR and H₂O₂-mediated DNA damage

• Published in: European food research & technology Vol. 226; no. 1-2; pp. 71 - 79
• Main Authors: Ahn, Gin-Nae, Kim, Kil-Nam, Cha, Seon-Heui, Song, Choon-Bok, Lee, Jehee, Heo, Moon-Soo, Yeo, In-Kyu, Lee, Nam-Ho, Jee, Young-Heun, Kim, Jin-Soo, Heu, Min-Soo, Jeon, You-Jin
• Format: Journal Article
• Language: English
• Published: Heidelberg Berlin/Heidelberg : Springer-Verlag 01-11-2007; Berlin Springer; Springer Nature B.V
• Subjects: antioxidant activity; Antioxidants; Bioassays; Biological and medical sciences; Comet assay; Deoxyribonucleic acid; DNA; DNA damage; Ecklonia cava; Electron spin resonance (ESR); Electrons; Food industries; Free radicals; Fundamental and applied biological sciences. Psychology; Hydrogen peroxide; Lymphoma; Natural products; Phlorotannin; Spectrometry; Phlorotannin; Electron spin resonance (ESR); DNA; Antioxidant activity; DNA damage; EPR spectrometry; Ecklonia cava; Antioxidant; Damage; Free radical; Comet assay
• ISSN: 1438-2377; 1438-2385
• DOI: 10.1007/s00217-006-0510-y

The potential antioxidant activities of three phlorotannins (phloroglucinol, eckol and dieckol) purified from Ecklonia cava collected in Jeju Island were investigated to evaluate their potential value as the natural products for foods or cosmetic application. In this study, antioxidant activities were measured by electron spin resonance spectrometry (ESR) technique for scavenging effects of free radicals such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), alkyl, hydroxyl (HO[round bullet, filled]) and superoxide anion radical (O₂ [round bullet, filled]-) and by comet assay for protecting effects against H₂O₂-mediated DNA damage. The results show that all the phlorotannins have the potential DPPH, alkyl, hydroxyl and superoxide radical scavenging activities. Especially, eckol samples scavenged around 93% of DPPH at 0.25, 0.5, 1 mg/mL of concentrations and were higher than the other phlorotannins, such as phloroglucinol and dieckol samples. Also, protecting effects of the phlorotannins against H₂O₂-mediated DNA damage increased with increased concentrations of the samples in the L5178 mouse T-cell lymphoma cell lines (L5178Y-R). In conclusion, these results suggest that the three phlorotannins purified from E. cava have the potential inhibitory effect on H₂O₂-mediated DNA damage and harmful free radicals and can be used as antioxidants in cosmetic, foods and drug industry.