Effects of live attenuated and killed Salmonella vaccine on T-lymphocyte mediated immunity in laying hens

• Published in: Veterinary immunology and immunopathology Vol. 91; no. 1; pp. 39 - 44
• Main Authors: Babu, U, Scott, M, Myers, M.J, Okamura, M, Gaines, D, Yancy, H.F, Lillehoj, H, Heckert, R.A, Raybourne, R.B
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10-01-2003
• Subjects: Animals; CD4; CD4-CD8 Ratio; CD8; Chickens - immunology; Con A; Female; Flagella; Immunization; Lymphocyte Activation; Poultry Diseases - prevention & control; Proliferation; Salmonella enteritidis; Salmonella enteritidis - immunology; Salmonella Infections, Animal - prevention & control; Salmonella Vaccines - immunology; Splenic lymphocytes; T-Lymphocytes - immunology; Vaccines; Vaccines, Attenuated - immunology; αβ T-cells; γδ T-cells; Salmonella enteritidis; CD4; αβ T-cells; CD8; Con A; Splenic lymphocytes; Proliferation; Vaccines; Flagella; γδ T-cells
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/S0165-2427(02)00265-9

The impact of live and killed
Salmonella vaccines on cell-mediated immunity (CMI) was investigated in 18- and 32-week-old White Leghorn chickens, by assessing splenic lymphocyte proliferation, expression of IL-2 mRNA in concanavalin A (Con A) stimulated cells and flow cytometric analysis of cell subpopulations. Con A and
Salmonella enteritidis (SE) flagella induced proliferation of splenocytes were enhanced in the 18- and 32-week-old chickens treated with live vaccine, compared to the corresponding control chickens. Among the killed vaccine treated birds, Con A-mediated response was higher in the 18-week-old chickens compared to the corresponding control birds. Increased proliferation was accompanied by increased CD4 and reduced CD8 and γδ T-lymphocytes in the 18-week-old live vaccine treated chickens. Relative expression of IL-2 mRNA in Con A-stimulated splenocytes from 18-week-old birds was not affected by vaccine treatment. Overall, live vaccine was more effective in increasing the lymphocyte proliferation to Con A as well as SE antigen. This enhanced CMI may prove beneficial in protecting chickens against SE infection.