Interferon gamma and lipopolysaccharide upregulate TNF-related apoptosis-inducing ligand expression in murine microglia

• Published in: Immunology letters Vol. 85; no. 3; pp. 271 - 274
• Main Authors: Genc, Sermin, Kizýldag, Sefa, Genc, Kursad, Ates, Halil, Atabey, Nese
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 03-02-2003
• Subjects: Animals; Apoptosis Regulatory Proteins; Flow Cytometry; Immunoblotting; Interferon-gamma - metabolism; Lipopolysaccharides - metabolism; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mice; Microglia; Microglia - metabolism; Murine; N9 cell line; Neurodegeneration; Precipitin Tests; RNA, Messenger; TNF-Related Apoptosis-Inducing Ligand; TRAIL; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Up-Regulation - physiology; TRAIL; N9 cell line; Neurodegeneration; Murine; Microglia
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/S0165-2478(02)00245-6

In this study, it is reported that neonatal murine microglia and N9 murine microglial cell line express tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). TRAIL protein and mRNA expression in murine microglia greatly upregulate upon stimulation with interferon gamma (IFNγ) or lipopolysaccharide (LPS) as revealed by immunoprecipitation-immunoblotting, reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry techniques. IFNγ and LPS act synergistically to induce TRAIL expression on both translational and transcriptional levels. The upregulated microglial TRAIL in inflammatory conditions may involve in the cytotoxic effect of these cells and play a role in neurodegenerative processes.