Functional analysis of the grapevine virus a genome

• Published in: Virology (New York, N.Y.) Vol. 306; no. 1; pp. 42 - 50
• Main Authors: Galiakparov, N, Tanne, E, Sela, Ilan, Gafny, R
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-02-2003
• Subjects: Capsid Proteins - genetics; Capsid Proteins - metabolism; Genome, Viral; Grapevine; GVA; Infectious clone; Mutagenesis; Mutation; Nicotiana - virology; Open Reading Frames; Plant Diseases - virology; Plant Viral Movement Proteins; Plant Viruses - genetics; Plant Viruses - pathogenicity; Plant Viruses - physiology; RNA Viruses - genetics; RNA Viruses - pathogenicity; RNA Viruses - physiology; Viral Proteins - genetics; Viral Proteins - metabolism; Vitis - virology; Vitiviruses; Infectious clone; Vitiviruses; GVA; Mutagenesis; Grapevine
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/S0042-6822(02)00019-3

Grapevine virus A (GVA) carries five open reading frames (ORFs). Only the coat protein ORF has been experimentally identified as such; the roles of some of the other ORFs have been deduced by sequence homology to known genes
(Minafra et al., 1997). The construction of a full-length, infectious clone of GVA has been previously reported
(Galiakparov et al., 1999). In an attempt to experimentally define the role of the various genes of GVA, we utilized the infectious clone, inserted mutations in every ORF, and studied the effect on viral replication, gene expression, symptoms and viral movement. Mutations in ORF 1 abolished RNA replication. Mutations in ORF 2 did not affect any of the aforementioned parameters. Mutations in ORFs 3 and 4 restricted viral movement. Mutations in ORF 5 rendered the virus asymptomatic, and partially restricted its movement.