Effects of Cr Stress on Bacterial Community Structure and Composition in Rhizosphere Soil of Iris tectorum under Different Cultivation Modes

• Published in: Microbiology research Vol. 14; no. 1; pp. 243 - 261
• Main Authors: Wei, Zhao, Sixi, Zhu, Baojing, Gu, Xiuqing, Yang, Guodong, Xia, Baichun, Wang
• Format: Journal Article
• Language: English
• Published: Perugia MDPI AG 01-03-2023
• Subjects: 16S rRNA sequencing technology; Abundance; Bacteria; Bioremediation; Chromium; Community structure; Cr stress; Cultivation; Environmental restoration; Experiments; Heavy metals; Industrial development; Iris tectorum; Microenvironments; Microorganisms; Network analysis; Next-generation sequencing; Nitrates; Nitrogen; Nutrients; phytoremediation; Plant diversity; Plant growth; Redundancy; Rhizosphere; rhizosphere bacterial community; Rhizosphere microorganisms; rRNA 16S; Seeds; Soil contamination; Soil ecology; Soil improvement; Soil microorganisms; Soil pollution; Soils; Synergistic effect; Textiles; Wetlands; United States > US
• ISSN: 2036-7481; 2036-7481
• DOI: 10.3390/microbiolres14010020

With the rapid development of industry, Cr has become one of the major heavy metal pollutants in soil, severely impacting soil microecology, among which rhizosphere microorganisms can improve the soil microenvironment to promote plant growth. However, how rhizosphere bacterial communities respond to Cr stress under different cultivation modes remains to be further studied. Therefore, in this study, a greenhouse pot experiment combined with 16S rRNA high-throughput sequencing technology was used to study the effects of Cr stress at 200 mg kg−1 on the bacterial community structure and diversity in the rhizosphere soil of Iris tectorum under different cultivation modes. The results showed that the rhizosphere bacterial community diversity index (Shannon and Simpson) and abundance index (Ace and Chao) increased significantly with wetland plant diversity under Cr stress. Moreover, the bacterial community changed by 20.1% due to the addition of Cr, further leading to a 15.9% decrease in the common species of the bacterial community, among which Proteobacteria, Actinobacteria, Chloroflexi, and Acidobacteriota accounted for more than 74.8% of the total sequence. However, with the increase in plant diversity, the abundance of rhizosphere-dominant bacteria and plant growth-promoting bacteria communities increased significantly. Meanwhile, the symbiotic network analysis found that under the two cultivation modes, the synergistic effect between the dominant bacteria was significantly enhanced, and the soil microenvironment was improved. In addition, through redundancy analysis, it was found that C, N, and P nutrients in uncontaminated soil were the main driving factors of bacterial community succession in the rhizosphere of I. tectorum, and Cr content in contaminated soil was the main driving factor of bacterial community succession in I. tectorum rhizosphere. In summary, the results of this study will provide a basis for the response of the rhizosphere bacterial community to Cr and the interaction between wetland plants and rhizosphere bacteria in the heavy metal restoration of wetland plants under different cultivation modes.