Fluorimetric determination of some thiol compounds in their dosage forms

• Published in: Farmaco (Società chimica italiana : 1989) Vol. 57; no. 8; pp. 625 - 629
• Main Authors: Al-Ghannam, Sh.M, El-Brashy, A.M, Al-Farhan, B.S
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier SAS 01-08-2002; Elsevier Science
• Subjects: 1,2-Naphthoquinone-4-sulfonic acid; Analysis; Biological and medical sciences; Captopril; d-penicillamine; Dosage Forms; General pharmacology; Medical sciences; N-acetylcysteine; Pharmacology. Drug treatments; Spectrometry, Fluorescence - methods; Sulfhydryl Compounds - analysis; Sulfhydryl Compounds - chemistry; 1,2-Naphthoquinone-4-sulfonic acid; Captopril; d-penicillamine; Dosage forms; N-acetylcysteine; Thiol; Chemical analysis; Acetylcysteine; Penicillamine; Fluorescence spectrometry; Sulfur containing aminoacid; Mucolytic; Dosage form; Antihypertensive agent; Tablet; Hard capsule; Antirheumatic agent; ACE inhibitor; Quantitative analysis
• ISSN: 0014-827X; 1879-0569
• DOI: 10.1016/S0014-827X(02)01223-5

A simple fluorimetric procedure was adopted for determination of three pharmaceutical compounds containing thiol groups namely, captopril,
d-penicillamine and
N-acetylcysteine. In this method, the drugs are treated with 1,2-naphthoquinone-4-sulfonic acid. The latter is reduced to 1,2-dihydroxynaphthalene-4-sulfonic acid which has a maximum fluorescence intensity at 480/318 nm (
λEm/Ex). The method is sensitive to 0.5–4.5 μg ml
−1 with minimum detectability 0.05 μg ml
−1 (
S/
N=2), and has been applied to determine these three thiols in their dosage forms. The results obtained are compared favourably with those obtained by their pharmacopeial methods.