pH-responsive magnetic graphene oxide composite as an adsorbent with high affinity for rapid capture of nucleosides

• Published in: Mikrochimica acta (1966) Vol. 190; no. 9; p. 365
• Main Authors: Yin, Huawen, Yuan, Yue, Xin, Ling, Hang, Qian, Zhao, Longshan, Qin, Feng, Xiong, Zhili
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-09-2023; Springer; Springer Nature B.V
• Subjects: Adenosine; Adsorbents; Adsorption; Analysis; Analytical Chemistry; Biological properties; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Design optimization; Graphene; Graphite; Hydrogen-ion concentration; Linearity; Magnetic properties; Microengineering; Nanochemistry; Nanotechnology; Nucleosides; Original Paper; Response surface methodology; Solid phases; Polyethyleneimine; Boron affinity; Magnetic dispersive micro-solid-phase extraction; Ultrahigh-performance liquid chromatography
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-023-05945-2

A novel pH-responsive magnetic graphene oxide composite (MGO@PEI-BA) is proposed for the first time as an adsorbent for the rapid capture and detection of nucleosides (cytidine, uridine, guanosine, and adenosine). The morphology, structure, and magnetic properties of the composite were evaluated using various characterization techniques. The results indicated that the composite was successfully fabricated. A series of parameters that affect extraction and elution were optimized through one-factor-at-a-time and Box-Behnken design of response surface methodology (BBD-RSM). The unique layered structures and easily accessible active sites of the composite facilitated molecular transport, resulting in instantaneous equilibrium of nucleosides adsorption within 5 min. Based on this study, a magnetic dispersive micro-solid-phase extraction (MD-μ-SPE) method assisted by the MGO@PEI-BA was developed in combination with UHPLC-UV analysis for the determination of nucleosides in rat urine. Under the optimum conditions, a wide linear range (10–2000 ng mL
−1
), good linearity (
r
> 0.99), low detection limits (1–3 ng mL
−1
), low relative standard deviations (RSDs ≤ 3.9%), and satisfactory recoveries (82.7–96.3%) were achieved. These results demonstrate that the MGO@PEI-BA is an excellent adsorbent for extracting nucleosides from biological samples.
Graphical Abstract