Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 765; no. 1; pp. 107 - 111
• Main Authors: Viant, Mark R, Rosenblum, Eric S, Tjeerdema, Ronald S
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05-12-2001
• Subjects: Animals; Arginine - analogs & derivatives; Arginine - analysis; Calibration; Chromatography, High Pressure Liquid - methods; Mollusca - chemistry; Organophosphorus Compounds - analysis; Phosphoarginine; Reproducibility of Results; Phosphoarginine
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(01)00428-5

A rapid high-performance liquid chromatography method for the determination of phosphoarginine (PArg) in invertebrate tissue has been redeveloped and validated. The method employs a reversed-phase amino column and a KH
2PO
4–acetonitrile mobile phase. PArg peak identity was confirmed by comparison with a known standard and via enzymatic conversion. Additionally linear calibration data, low intra-assay variability (<4%), and a detection limit of 5 pmol were determined. The method was demonstrated using PArg extracted from red abalone (
Haliotis rufescens) adductor muscle. Validation of the extraction procedure was also completed, including the measurement of a 100.2±0.9% extraction efficiency.