Cellular uptake of N-methylpyrrole/ N-methylimidazole polyamide-dye conjugates

• Published in: Bioorganic & medicinal chemistry Vol. 10; no. 10; pp. 3313 - 3318
• Main Authors: Belitsky, Jason M, Leslie, Stephanie J, Arora, Paramjit S, Beerman, Terry A, Dervan, Peter B
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-10-2002; Elsevier Science
• Subjects: Biological and medical sciences; Boron Compounds - pharmacokinetics; Cell Compartmentation; Cell Nucleus - metabolism; Cytoplasm - metabolism; Fluorescent Dyes - pharmacokinetics; General pharmacology; Humans; Imidazoles - pharmacokinetics; Medical sciences; Microscopy, Confocal; Miscellaneous; Nylons - pharmacokinetics; Pharmacology. Drug treatments; Pyrroles - pharmacokinetics; T-Lymphocytes - cytology; T-Lymphocytes - metabolism; T-Lymphocytes - ultrastructure; Tumor Cells, Cultured; Imidazole derivatives; Nylon; Ligand; Insecta; Spodoptera frugiperda; Confocal microscopy; Uptake; Pyrrole derivatives; Biological fixation; Amine borane; Cell line; Synthetic product; Arthropoda; DNA; Lepidoptera; Boron Organic compounds; Noctuidae; Mammary gland; Invertebrata; Conjugated compound; Cytoplasm
• ISSN: 0968-0896; 1464-3391
• DOI: 10.1016/S0968-0896(02)00204-3

The cellular uptake and localization properties of DNA binding
N-methylpyrrole/
N-methylimidazole polyamide–dye conjugates in a variety of living cells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide–dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.
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