A cytochrome P450 gene involved in methidathion resistance in Amblyseius womersleyi Schicha (Acari: Phytoseiidae)

• Published in: Pesticide biochemistry and physiology Vol. 88; no. 3; pp. 337 - 345
• Main Authors: Sato, Mário Eidi, Tanaka, Toshiharu, Miyata, Tadashi
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-07-2007; Elsevier
• Subjects: Acari; acaricide resistance; Amblyseius; Amblyseius womersleyi; Biological and medical sciences; biological control agents; Control; CYP4; cytochrome P-450; developmental stages; enzyme activity; enzymes; Fundamental and applied biological sciences. Psychology; Gene expression; genes; Insecticide resistance; methidathion; monooxygenase; Monooxygenase activity; natural enemies; Phytopathology. Animal pests. Plant and forest protection; Phytoseiidae; Predaceous mite; Protozoa. Invertebrates; reverse transcriptase polymerase chain reaction; strain differences; strains; Monooxygenase activity; Predaceous mite; Insecticide resistance; Gene expression; CYP4; Insecticide; Acari; Enzymatic activity; Gene; Pesticide resistance; Gamasida; Parasitiformes; Enzyme; Cytochrome P450; Pesticides; Oxygenase; Arachnida; Sensitivity resistance; Arthropoda; Organophosphorus compounds; Oxidoreductases; Methidathion; Predator; Invertebrata; Phytoseiidae
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2007.01.010

This study reports on a CYP4 gene possibly involved in methidathion resistance in
Amblyseius womersleyi Schicha. This is the first report on a CYP4 gene from a phytoseiid mite. Four different CYP4 genes (
CYP4-a,
CYP4-b,
CYP4-c, and
CYP4-d) were detected in this predaceous mites. No differences in CYP4 expression levels were detected for the strains Ishigaki S, Kanaya S (methidathion susceptible strains) and Kanaya R (methidathion resistant strain), using RT-PCR method for
CYP4-a,
CYP4-b, and
CYP4-c. In the case of
CYP4-d, the expression level in Kanaya R was significantly higher than in susceptible strains. Studies using RT-PCR indicated that
CYP4-d gene is expressed in all stages of
A. womersleyi, with a little higher expression in protonymph, deutonymph and adult, in comparison to egg and larva. Significant correlation between
CYP4-d expression and monooxygenase activity was observed for the different strains and life stages of
A. womersleyi.