Gastric smooth muscle contractility changes in the esophageal atresia rat model: an in vitro study

• Published in: Journal of Pediatric Surgery Vol. 38; no. 9; pp. 1366 - 1370
• Main Authors: Tugay, Melih, Yildiz, Fürüzan, Utkan, Tijen, Sarıoglu, Yusuf, Gacar, Nejat
• Format: Book Review Journal Article
• Language: English
• Published: Philadelphia, PA Elsevier Inc 01-09-2003; Elsevier
• Subjects: Adriamycin; Animals; Biological and medical sciences; Disease Models, Animal; Doxorubicin; Esophageal atresia; Esophageal Atresia - chemically induced; Esophageal Atresia - physiopathology; Esophagus; Fetus; gastric fundus; Gastric Fundus - physiopathology; Gastroenterology. Liver. Pancreas. Abdomen; General aspects; in vitro; In Vitro Techniques; Malformations; Medical sciences; Muscle Contraction; Muscle, Smooth - physiopathology; rat; Rats; Rats, Sprague-Dawley; Esophageal atresia; rat; Adriamycin; gastric fundus; in vitro; Stomach; Animal model; Pediatrics; Rat; Digestive system; Esophageal disease; Rodentia; Contractility; Model study; Smooth muscle; Change; In vitro; Medicine; Vertebrata; Mammalia; Digestive diseases
• ISSN: 0022-3468; 1531-5037
• DOI: 10.1016/S0022-3468(03)00397-X

Purpose:
The aim of the study was to investigate the gastric smooth muscle reactivity in the Adriamycin-induced esophageal atresia (EA) rat model.
Methods:
The fetuses were divided into 3 groups. The control group was exposed to saline. The second group was comprised of fetuses that were exposed to Adriamycin but did not have EA (Adriamycin-no-EA group). The third group was comprised of fetuses that were exposed to Adriamycin and had EA (Adriamycin-EA group). Gastric fundus strips were studied in vitro for their contractile response to receptor activation in the 3 groups.
Results:
Contractile responses of gastric smooth muscle to carbachol and KCl were increased in the Adriamycin-EA group compared with the Adriamycin-no-EA group. Also serotonin-induced contractile response in the Adriamycin-EA group decreased compared with the Adriamycin-no-EA group. Relaxation of gastric smooth muscle strips to isoproterenol was comparably unaffected in the Adriamycin-EA and Adriamycin-no-EA groups. Likewise, no change in the response to agonist studies was observed between the control and Adriamycin-no-EA groups. The relaxant response to papaverine was not different in the 3 groups.
Conclusions:
This study found changes of receptor-dependent and receptor-independent contraction of the gastric fundus smooth muscle in the fetuses with EA. Therefore, impaired contractile responses may be, at least in part, a contributing factor in the abnormal gastric motility seen in EA.