Fluorescent probe detect distinguishly sulfite/hydrogen sulfide and thiol via two emission channels in vivo

• Published in: Sensors and actuators. B, Chemical Vol. 277; pp. 647 - 653
• Main Authors: Xie, Xixi, Yin, Caixia, Yue, Yongkang, Chao, Jianbin, Huo, Fangjun
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 20-12-2018; Elsevier Science Ltd
• Subjects: Bio-thiols; Channels; Coumarin; Distinguishly; Emission; Emissions; Fluorescence; Fluorescent indicators; Fluorescent probes; Hydrogen sulfide; In vivo imaging; Metabolites; Rodents; Sulfite; Sulfur dioxide; Thiols; Sulfite; Fluorescent probes; Distinguishly; Bio-thiols; In vivo imaging
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2018.09.064

[Display omitted]
•A probe for detecting distinguishly sulfite/hydrogen sulfide and thiol via two emission channels was obtained.•The recognition process showed different reaction properties between sulfite/hydrogen sulfide and thiol.•The probe is low toxic and could be applied in bio-imaging and live mouse tissues.
For SO2, another Cys metabolite, to simultaneously distinguish SO2 over thiols are still a challenge. Now we have successfully synthesized a novel coumarin-based turn-on fluorescent probe that could detect distinguishingly sulfite/hydrogen sulfide over bio-thiols through whether or not acryloyl group is used as a self-immolative linker. The detection limit of probe 1 for Cys, Hcy, GSH, HS− and SO32- were found to be ∼0.23, 1.05, 0.46, 0.52 and 0.16 μM, respectively. Such a probe could detect H2S/SO32− and bio-thiols via two emission channels in B16 cells, and possessed the appropriate ability to detect exogenous H2S in living mouse. These specific responses demonstrate that probe 1 has the potential to reflect the metabolic process of cysteine.