Tetrodotoxin and its analogues profile in nemertean species from the sea of Japan

• Published in: Toxicon (Oxford) Vol. 156; pp. 48 - 51
• Main Authors: Vlasenko, A.E., Velansky, P.V., Chernyshev, A.V., Kuznetsov, V.G., Magarlamov, T. Yu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15-12-2018
• Subjects: Animals; Aquatic Organisms - chemistry; Aquatic Organisms - metabolism; Chromatography, High Pressure Liquid - methods; HPLC-MS/MS; Invertebrates - chemistry; Invertebrates - metabolism; Japan; Nemerteans; Pacific Ocean; Tandem Mass Spectrometry - methods; Tetrodotoxin; Tetrodotoxin - analogs & derivatives; Tetrodotoxin - analysis; Tetrodotoxin - chemistry; Tetrodotoxin - metabolism; Tetrodotoxin analogues; TTX; Japan; Pacific Ocean; Tetrodotoxin; TTX; Nemerteans; HPLC-MS/MS; Tetrodotoxin analogues
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2018.11.006

For the first time search for tetrodotoxin (TTX) and its analogues in the extracts of nemerteans using HPLC-MS/MS was performed. TTX analogues were detected in two nemertean species in addition to TTX: 7 analogues were detected in the extract of Cephalothrix simula, 3 analogues - in the extract 11-norTTX of Kulikovia manchenkoi. Presence of 5-deoxyTTX, 11-deoxyTTX, 5,6,11-trideoxyTTX and −6(R)-ol in nemerteans was shown for the first time.
•TTX and its analogues were first detected in ribbon worms via HPLC-MS/MS.•Four analogues of TTX were revealed in nemertean species for the first time.•5-deoxyTTX (K. manchenkoi) and 5,6,11-trideoxyTTX (C. simula) are dominant toxins.