High-resolution banding in chromosomes of B lymphoblastoid cells and cultured skin fibroblasts

High-resolution banded chromosomes were obtained in Epstein-Barr virus-transformed lymphoblastoid cells and cultured skin fibroblasts by aphidicolin synchronization, followed by inhibition of chromosome condensation by ethidium bromide. With this method, the chromosomes of 33.3% of lymphoblastoid ce...

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Bibliographic Details
Published in:	Cytogenetics and cell genetics Vol. 56; no. 3-4; p. 212
Main Authors:	Kuwano, A, Kajii, T
Format:	Journal Article
Language:	English
Published:	Switzerland 01-01-1991
Subjects:	Aphidicolin B-Lymphocytes - ultrastructure Cells, Cultured Chromosome Banding - methods Diterpenes - pharmacology Ethidium - pharmacology Fibroblasts - ultrastructure Humans In Vitro Techniques
Online Access:	Get more information
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Summary:	High-resolution banded chromosomes were obtained in Epstein-Barr virus-transformed lymphoblastoid cells and cultured skin fibroblasts by aphidicolin synchronization, followed by inhibition of chromosome condensation by ethidium bromide. With this method, the chromosomes of 33.3% of lymphoblastoid cells and 48.7% of skin fibroblasts were high-resolution banded, and their mitotic indices were 1.5 and 2.7 times higher than those of control cultures.
ISSN:	0301-0171
DOI:	10.1159/000133091