Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography

A quick and direct method for measuring tocopherols (α, β+γ and δ) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (α-tocopherol...

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Bibliographic Details
Published in:	Journal of Chromatography A Vol. 881; no. 1; pp. 251 - 254
Main Authors:	Gimeno, E, Castellote, A.I, Lamuela-Raventós, R.M, de la Torre, M.C, López-Sabater, M.C
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 09-06-2000
Subjects:	Chromatography, High Pressure Liquid - methods Plant Oils - chemistry Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet Tocopherols Vitamin E - analysis Vitamins Tocopherols Oils Food analysis Vitamins
Online Access:	Get full text
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Summary:	A quick and direct method for measuring tocopherols (α, β+γ and δ) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (α-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol–water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9673
DOI:	10.1016/S0021-9673(00)00219-3