SDE5, a putative RNA export protein, participates in plant innate immunity through a flagellin-dependent signaling pathway in Arabidopsis

• Published in: Scientific reports Vol. 7; no. 1; pp. 9859 - 11
• Main Authors: Uddin, Mohammad Nazim, Akhter, Salina, Chakraborty, Rupak, Baek, Ji Hyeong, Cha, Joon-Yung, Park, Su Jung, Kang, Hunseung, Kim, Woe-Yeon, Lee, Sang Yeol, Mackey, David, Kim, Min Gab
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29-08-2017; Nature Publishing Group
• Subjects: 38; 38/1; 38/77; 631/449; 631/449/2661/2666; 82; Arabidopsis; Arabidopsis - genetics; Arabidopsis - immunology; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Bacteria; Data processing; Disease Resistance - genetics; Disease Resistance - immunology; Flagellin; Flagellin - metabolism; Gene Expression Regulation, Plant; Humanities and Social Sciences; Immunity, Innate - genetics; Innate immunity; Methyl jasmonate; mRNA; multidisciplinary; Mutation; Phenotype; Plants, Genetically Modified; Protein transport; RNA Transport; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA-mediated interference; Salicylic acid; Science; Science (multidisciplinary); Signal Transduction; siRNA; Tomatoes; Transgenic plants
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-07918-x

In eukaryotes, RNA silencing, mediated by small interfering RNAs, is an evolutionarily widespread and versatile silencing mechanism that plays an important role in various biological processes. Increasing evidences suggest that various components of RNA silencing pathway are involved in plant defense machinery against microbial pathogens in
Arabidopsis thaliana
. Here, we show genetic and molecular evidence that Arabidopsis
SDE5
is required to generate an effective resistance against the biotrophic bacteria
Pseudomonas syringae
pv.
tomato
DC3000 and for susceptibility to the necrotrophic bacteria
Erwinia caratovora
pv.
caratovora
.
SDE5
, encodes a putative mRNA export factor that is indispensable for transgene silencing and the production of
trans
-acting siRNAs.
SDE5
expression is rapidly induced by exogenous application of phytohormone salicylic acid (SA), methyl jasmonate (MeJA), phytopathogenic bacteria, and flagellin. We further report that SDE5 is involved in basal plant defense and mRNA export. Our genetic data suggests that SDE5 and Nonexpressor of PR Gene1 (NPR1) may contribute to the same SA-signaling pathway. However,
SDE5
over-expressing transgenic plant exhibits reduced defense responsive phenotype after flagellin treatment. Taken together, these results support the conclusion that SDE5 contributes to plant innate immunity in
Arabidopsis
.