Measurement protocol for planar lipid bilayer viscoelastic properties

• Published in: IEEE transactions on dielectrics and electrical insulation Vol. 16; no. 5; pp. 1236 - 1242
• Main Authors: Sabotin, I., Lebar, A.M., Miklavcic, D., Kramar, P.
• Format: Journal Article
• Language: English
• Published: New York IEEE 01-10-2009; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Bioelectric phenomena; biomechanics; Biomembranes; Breakdown; Breakdown voltage; capacitance measurement; dielectric breakdown; Elasticity; electric field effects; Electric potential; Electric variables measurement; electromechanical effects; Lipidomics; Lipids; Membranes; Modules; Protocols; Pulse measurements; Surface tension; Viscoelasticity; Viscosity; Voltage; Voltage measurement
• ISSN: 1070-9878; 1558-4135
• DOI: 10.1109/TDEI.2009.5293933

This paper describes how to estimate planar lipid bilayer's elasticity module E and surface tension sigma by means of measuring its breakdown voltage and using Dimitrov's viscoelastic model of electric field-induced breakdown of lipid bilayers. Planar lipid bilayers (BLMs) were made of two components: 1-palmitoyl 2-oleoyl phosphatidylcholine (POPC) and 1-palmitoyl 2-oleoyl phosphatidylserine (POPS) in five different compositions. Folding method for forming planar lipid bilayers in the salt solution of 100 mM KCl was used. Breakdown voltages U br and membrane life times t br were measured by means of applying linear rising voltage signals of seven different slopes. Specific capacitances c BLM of bilayers were measured with charge pulse method. Then Dimitrov's viscoelastic model was fitted to measured data allowing for estimation of elasticity module and surface tension of the lipid bilayer. Our results show that onecomponent bilayers composed from POPS were more stable and thus having higher breakdown voltages and elasticity moduli then bilayers composed of POPC. Surface tension values were similar regardless of the membrane composition. Values of the elasticity (E) and surface tension (sigma) are comparable to those published in the literature. We conclude that the protocol used, though time consuming, is an alternative to other methods used for determination of bilayer's mechanical properties.