ShcD interacts with TrkB via its PTB and SH2 domains and regulates BDNF-induced MAPK activation

• Published in: BMB reports Vol. 43; no. 7; pp. 485 - 490
• Main Authors: You, Yuangang, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Li, Weiqi, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Gong, Yanhua, Medical College of Chinese People's Armed Police Force, Tianjin, China, Yin, Bin, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Qiang, Boqin, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Yuan, Jiangang, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Peng, Xiaozhong, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
• Format: Journal Article
• Language: English
• Published: Korea (South) 생화학분자생물학회 01-07-2010
• Subjects: Brain-Derived Neurotrophic Factor - metabolism; Cell Line; Humans; Immunoprecipitation; MAPK; Mitogen-Activated Protein Kinases - metabolism; Phosphorylation; Protein Structure, Tertiary; PROTEINAS; PROTEINE; PROTEINS; PTB domain; Receptor, trkB - metabolism; SH2 domain; Shc Signaling Adaptor Proteins - analysis; Shc Signaling Adaptor Proteins - metabolism; ShcD; Signal Transduction; src Homology Domains; TrkB receptor; Two-Hybrid System Techniques; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2010.43.7.485

Neurotrophins regulate many aspects of neuronal function through activation of the high affinity Irk receptors. Shc family proteins are implicated in the coupling of RTK to the Ras/mitogen-activated protein kinase signaling cascade. Here we report that the fourth Shc family member, ShcD, associates with TrkB receptor and regulates BDNF-induced MAPK activation. Yeast two-hybrid assay and Co-IP experiments demonstrate ShcD interacts with TrkB in a kinase-activity-dependent manner. Confocal analysis shows ShcD cololizes well with TrkB in transfected 293T cells. Subsequent mapping experiments and mutational analysis indicate that both PTB and SH2 domains are capable of binding to TrkB and PTB domain binds to TrkB NPQY motif. Furthermore, ShcD is involved in BDNF-induced MAPK activation. In summary, we demonstrate that ShcD is a substrate of TrkB and mediates TrkB downstream signaling pathway.