Novel oligosaccharides obtained by bacteriophage degradation of the polysaccharide from Klebsiella serotype K26

Bacteriophage phi 26 was used to depolymerize the polysaccharide of Klebsiella K26, yielding three oligosaccharides. The major product was the heptasaccharide repeating unit, with one of the minor products being the fourteen-sugar oligosaccharide corresponding to two repeating units. The other minor...

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Bibliographic Details
Published in:	Carbohydrate research Vol. 144; no. 2; p. 251
Main Authors:	Di Fabio, J L, Karunaratne, D N, Dutton, G G
Format:	Journal Article
Language:	English
Published:	Netherlands 01-12-1985
Subjects:	Bacteriophages - metabolism Carbohydrate Conformation Carbohydrate Sequence Gas Chromatography-Mass Spectrometry Klebsiella - immunology Magnetic Resonance Spectroscopy Methylation Oligosaccharides - isolation & purification Polysaccharides, Bacterial
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Summary:	Bacteriophage phi 26 was used to depolymerize the polysaccharide of Klebsiella K26, yielding three oligosaccharides. The major product was the heptasaccharide repeating unit, with one of the minor products being the fourteen-sugar oligosaccharide corresponding to two repeating units. The other minor product was unusual since it was a hexasaccharide devoid of the terminal, pyruvate-containing galactose unit present in the side chain of the normal repeating unit. Phage phi 26 was shown to act as a beta-galactosidase, and hence it may have the ability to remove the terminal beta-galactose residue in the side chain.
ISSN:	0008-6215
DOI:	10.1016/S0008-6215(00)90673-6