Preeclampsia: The effects of serum on endothelial cell prostacyclin, endothelin, and cell membrane integrity

• Published in: American journal of obstetrics and gynecology Vol. 174; no. 2; pp. 737 - 743
• Main Authors: Zammit, Vivienne C., Whitworth, Judith A., Brown, Mark A.
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Mosby, Inc 01-02-1996; Elsevier
• Subjects: 6-Ketoprostaglandin F1 alpha - biosynthesis; Biological and medical sciences; Blood; Cell Membrane - physiology; Cells, Cultured; Chromium Radioisotopes - metabolism; Culture Media; cytotoxicity; Diseases of mother, fetus and pregnancy; endothelin; Endothelins - biosynthesis; Endothelium; Endothelium, Vascular - metabolism; Endothelium, Vascular - ultrastructure; Epoprostenol - biosynthesis; Female; Gynecology. Andrology. Obstetrics; Humans; Medical sciences; Pre-Eclampsia - blood; preeclampsia; Pregnancy; Pregnancy. Fetus. Placenta; prostacyclin; Umbilical Veins; endothelin; preeclampsia; prostacyclin; cytotoxicity; Endothelium; Human; Cell culture; Endothelial cell; Endothelin; Pregnancy disorders; Prostaglandin I2; Prostaglandin; Pathogenesis; Arachidonic acid derivatives; Peptide hormone; In vitro; Pregnancy toxemia; Preeclampsia; Plasma membrane; Serum; Vasoconstrictor agent
• ISSN: 0002-9378; 1097-6868
• DOI: 10.1016/S0002-9378(96)70458-1

OBJECTIVE: Our purpose was to determine whether serum from women with preeclampsia or gestational hypertension (1) decreased endothelial cell prostacyclin, (2) increased endothelial cell endothelin, and (3) caused endothelial cell damage.
STUDY DESIGN: Production of 6-keto-prostaglandin F
1
α
and endothelin by cultured endothelial cells was measured after 48 hours' incubation with sera from 23 nonpregnant women, 23 normal pregnant women, 12 women with preeclampsia, and 11 women with gestational hypertension. Structural damage of endothelial cells was assessed by a chromium release assay.
RESULTS: Serum from normal pregnant women induced more endothelial prostacyclin but less endothelin than did serum from nonpregnant women (
p < 0.05). No difference was found between normal pregnant and hypertensive pregnant women for prostacyclin production, but serum of preeclamptic women induced less endothelin production than did that of normal pregnant women (
p < 0.05). Chromium 51 release by endothelial cells was similar between normal pregnant and hypertensive pregnant groups.
CONCLUSIONS: Serum from preeclamptic women stimulates less endothelin production than does serum from normal pregnant women but does not alter prostacyclin production and is not cytotoxic to endothelial cells after short-term incubation. (A
M J O
BSTET G
YNECOL 1996;174:737-43.)