Enrichment and fluorogenic labelling of 5-formyluracil in DNA

Enrichment and fluorogenic labelling of 5-formyluracil in DNA

Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated -phenylenediamine directly tethe...

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Bibliographic Details
Published in:Chemical science (Cambridge) Vol. 8; no. 6; pp. 4505 - 4510
Main Authors: Liu, Chaoxing, Wang, Yafen, Zhang, Xiong, Wu, Fan, Yang, Wei, Zou, Guangrong, Yao, Qian, Wang, Jiaqi, Chen, Yuqi, Wang, Shaoru, Zhou, Xiang
Format: Journal Article
Language:English
Published: England 01-06-2017
Subjects:
Deoxyribonucleic acid
Enrichment
Fluorescence
Labelling
Plugs
Reagents
Switches
Synthesis
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Summary:Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated -phenylenediamine directly tethered to naphthalimide can switch on 5-formyluracil, under physiological conditions, which can then be used in cell imaging after exposure to γ-irradiation. In addition, its labelling property caused the polymerase extension to stop in the 5-formyluracil site, which gave us more information than the fluorescence did itself. The idea of detecting 5-formyluracil might be used in the synthesis of other modified diaminofluoresceins.
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ISSN:2041-6520
2041-6539
DOI:10.1039/c7sc00637c