Extraction and clean-up procedure for analysis of organochlorine pesticide residues in ethoxylated lanolin

• Published in: Farmaco (Società chimica italiana : 1989) Vol. 55; no. 9; pp. 637 - 640
• Main Authors: Polese, Luciana, Sannomiya, Miriam, de Oliveira Sader, Ana Paula, Lúcia Ribeiro, Maria
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier SAS 01-09-2000; Elsevier Science
• Subjects: Animals; Applied sciences; Biological and medical sciences; Chemical industry and chemicals; Cosmetics, toiletries; Domestic and cosmetic products toxicology; Ethoxylated lanolin; Exact sciences and technology; Gas chromatography; Hydrocarbons, Chlorinated; Insecticides - analysis; Lanolin - chemistry; Medical sciences; Organochlorine pesticides; Pesticide Residues - analysis; Sheep; Toxicology; Washing products. Cosmetics and toiletries. Perfumes; Gas chromatography; Ethoxylated lanolin; Organochlorine pesticides; Trace analysis; Cosmetic; Electron capture detector; Solid phase extraction; Chemical analysis; End group; Pesticides; Raw materials; Contamination; Ethylene oxide polymer; Sample preparation; Organochlorine compounds; Residue; Lanolin; Gas liquid chromatography; Quantitative analysis
• ISSN: 0014-827X; 1879-0569
• DOI: 10.1016/S0014-827X(00)00089-6

In the present study an evaluation was made of a method for the determination of organochlorine pesticide residues in ethoxylated lanolin. Samples were homogenized with Celite, transferred to chromatographic columns, prepacked with silica gel deactivated to 10%. The pesticide elution was processed with
n-hexane–dichloromethane and the concentrated eluate was analyzed using gas–liquid chromatography (GC) with electron capture detection (ECD). The composition of the elution solvent was a significant factor for the recovery of the pesticides. Mean recoveries obtained for fortified samples ranged from 87 to 94% for
p,
p′-DDE, dieldrin, endrin,
p,
p′-DDD and
p,
p′-DDT. Optimization of the experimental conditions resulted in a small-scale method that combines extraction and cleanup in a single step.