The influence of temperature on the multiple separation of estrogenic steroids using mobile phases modified with β-cyclodextrin in high-performance liquid chromatography

The effect of temperature on the retention and multiple separation of six estrogenic steroids in reversed-phase liquid chromatography has been studied. Capacity factors (k′) of estriol, 17β-estradiol, 17α-estradiol, d-equilenin, equilin and estrone were measured using mobile phase modified with diff...

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Bibliographic Details
Published in:	Journal of pharmaceutical and biomedical analysis Vol. 15; no. 9; pp. 1281 - 1287
Main Authors:	Zarzycki, P.K., Wierzbowska, M., Lamparczyk, H.
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Amsterdam Elsevier B.V 01-06-1997 Elsevier Science
Subjects:	Analytical, structural and metabolic biochemistry beta-Cyclodextrins Biological and medical sciences Chromatography, High Pressure Liquid Cyclodextrin Cyclodextrins Estrogens Estrogens - isolation & purification Fundamental and applied biological sciences. Psychology Liquid chromatography Mobile phase additives Optimization criteria Other biological molecules Temperature Terpenes, steroids. Hormones Mobile phase additives Cyclodextrin Temperature Liquid chromatography Optimization criteria Estrogens Capacity factor Steroid Oligosaccharide Estrogen HPLC chromatography Environmental factor Mobile phase Retention time Optimization Separation method Reversed phase chromatography Concentration effect Sex steroid hormone Inclusion compound Quantitative analysis
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