Comparative proteomic analysis of the saliva of the Rhodnius prolixus, Triatoma lecticularia and Panstrongylus herreri triatomines reveals a high interespecific functional biodiversity

• Published in: Insect biochemistry and molecular biology Vol. 71; pp. 83 - 90
• Main Authors: Montandon, Carlos Emmanuel, Barros, Edvaldo, Vidigal, Pedro Marcus, Mendes, Maria Tays, Anhê, Ana Carolina Borella Marfil, de Oliveira Ramos, Humberto Josué, de Oliveira, Carlo José Freire, Mafra, Cláudio
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-04-2016
• Subjects: Animals; Arthropoda; Biodiversity; Electrophoresis, Gel, Two-Dimensional; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Insect Vectors - chemistry; Insect Vectors - genetics; Insect Vectors - metabolism; Mass Spectrometry; Panstrongylus; Panstrongylus - chemistry; Panstrongylus - genetics; Panstrongylus - metabolism; Panstrongylus herreri; Proteomics; Rhodnius - chemistry; Rhodnius - genetics; Rhodnius - metabolism; Rhodnius prolixus; Saliva - chemistry; Saliva - metabolism; Sialoproteomics; Triatoma - chemistry; Triatoma - genetics; Triatoma - metabolism; Triatoma lecticularia; Triatomines; Trypanosoma cruzi; Trypanosoma rangeli; Panstrongylus herreri; Rhodnius prolixus; Triatoma lecticularia; Triatomines; Sialoproteomics
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/j.ibmb.2016.02.009

Triatomines are hematophagous arthropods that transmit Trypanosoma cruzi and Trypanosoma rangeli. Feeding behavior and pathogen transmission is known to vary between the different species, and this characteristic is directly or indirectly dependent on the bioactive molecules of the saliva that facilitate the vector–host–parasite interaction. Here, we identify, characterize and compare the sialoproteomic (from the Greek sialo: saliva) repertoire of important species of the main triatomine genera in the Americas (Rhodnius prolixus, Triatoma lecticularia and Panstrongylus herreri) to better explain this interaction through two-dimensional electrophoresis and mass spectrometry. We identified 221 proteins, 69 from R. prolixus, 100 from T. lecticularia and 52 from P. herreri. We identified high abundance molecules with a great potential to modulate host defenses and homeostasis, highlighting Nitrophorin-4 (28.7%), Salivary lipocalin-5 (65.2%) and Putative triabin (20.5%) in R. prolixus, T. lecticularia and P. herreri, respectively. We also observed that only a single hypothetical protein is shared among three species, which was not functionally categorized. This study corroborates previous findings with R. prolixus, increasing the knowledge about this species with relevant proteomic information and comparisons with the other two targets of the study, T. lecticularia and P. herreri, for which no studies are available from a proteomics perspective.
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•221 proteins identified, of which 69 from Rhodnius prolixus, 100 from Triatoma lecticularia and 52 from Panstrongylus herreri.•Nitrophorin-4, Salivary lipocalin-5 and Putative triabin identified for R. prolixus, T. lecticularia and P. herreri, respectively.•Single protein in common identified for R. prolixus, T. lecticularia and P. herreri.•Relevant sialoproteomic comparison between R. prolixus, T. lecticularia and P. herreri.