Syntheses and immunologic properties of Escherichia coli O157 O-specific polysaccharide and Shiga toxin 1 B subunit conjugates in mice

Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157....

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Bibliographic Details
Published in:	Infection and immunity Vol. 67; no. 11; pp. 6191 - 6193
Main Authors:	KONADU, E, DONOHUE-ROLFE, A, CALDERWOOD, S. B, POZSGAY, V, SHILOACH, J, ROBBINS, J. B, SZU, S. C
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-11-1999
Series:	Note
Subjects:	Animals Antibodies, Bacterial - blood Bacterial Toxins - immunology Bacterial Vaccines - immunology Bacteriology Biological and medical sciences Escherichia coli Escherichia coli O157 - immunology Female Fundamental and applied biological sciences. Psychology HeLa Cells Humans lipopolysaccharides Mice Microbial Immunity and Vaccines Microbiology O Antigens - immunology polysaccharides Shiga toxin Shiga toxin 1 Shiga Toxins Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Conjugate - immunology Bivalent vaccine Immune response Antibody Toxoid Escherichia coli Rodentia Shiga-like toxin Toxin Vertebrata Mammalia Immunogenicity Mouse Lipopolysaccharide Bacteria Conjugated compound Humoral immunity Enterobacteriaceae Neutralization
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Summary:	Escherichia coli O157 is the major cause of diarrhea-associated hemolytic uremic syndrome (HUS). Strains causing HUS contain either Shiga toxin 1 (Stx1) or Stx2, or both. In adult volunteers, conjugate vaccines of detoxified lipopolysaccharide (LPS) elicited bactericidal antibodies to E. coli O157. Here, the detoxified LPS was conjugated with improved schemes to the nontoxic B subunit of Stx1. Mice injected with these bivalent conjugates elicited both bactericidal antibodies to E. coli O157 and neutralization antibodies to Stx1.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: National Institute of Child Health and Human Development, Room 424, Building 6, National Institutes of Health, Bethesda, MD 20892-2720. Phone: (301) 496-1185. Fax: (301) 402-9108. E-mail: scszu@helix.nih.gov.
ISSN:	0019-9567 1098-5522
DOI:	10.1128/iai.67.11.6191-6193.1999