Optimization of parameters for obtaining callus, suspension, and root cultures of meadowsweet (filipendula ulmaria) to isolate the largest number of biologically active substances with geroprotective properties

• Published in: Brazilian journal of biology Vol. 84; pp. e257074 - 14
• Main Authors: Dyshlyuk, S, Vesnina, A D, Dmitrieva, A I, Kozlova, O V, Prosekov, A Y
• Format: Journal Article
• Language: English
• Published: Brazil Instituto Internacional de Ecologia 2024
• Subjects: Aging; Antiinfectives and antibacterials; antimicrobial activity; Antimicrobial agents; Antioxidants; Biological activity; biologically active substances; BIOLOGY; Biomass; Callus; Cell culture; cell cultures in vitro; Cultivation; Culture media; Filipendula; Filipendula ulmaria; geroprotector; Growth rate; meadowsweet; Metabolites; Optimization; Parameters; Plant extracts; Plant Extracts - pharmacology; Raw materials; Secondary metabolites; Seeds; Russia; geroprotector; meadowsweet; biologically active substances; substâncias biologicamente ativas; ulmária; envelhecimento; cell cultures in vitro; cultura de células in vitro; aging; antimicrobial activity; atividade antimicrobiana; geroprotetor
• ISSN: 1519-6984; 1678-4375; 1678-4375
• DOI: 10.1590/1519-6984.257074

The study of biologically active substances-secondary metabolites of plants that exhibit geroprotective properties is an actual and popular direction in medicine to prevent early aging. This work aims to select the cultivation parameters for obtaining in vitro cell cultures of meadowsweet containing the largest amount of biologically active substances (BAS) for their further extraction as candidate substances for geroprotectors. To specify the effectiveness of the selected cell culture cultivation parameters, biomass growth for callus and root cultures, growth index, specific growth rate, and viability for suspension cultures was carried out. The study results made it possible to select the nutrient media for the cultivation of cell cultures of meadowsweet. It has been found that the greater the antioxidant activity of the extracts, the greater the antimicrobial properties it exhibits. In this study, cell cultures in vitro and alcohol extracts from the plant Filipendula ulmaria were considered as raw materials rich in candidate substances for geroprotectors. According to the data obtained, the plant is rich in hydroxybenzoic and salicylic acids, spireoside, avicularin, and hyperoside.