Antiparasitic potential of alternative treatments against larval stages of Lernaea cyprinacea

• Published in: Journal of parasitic diseases Vol. 45; no. 4; pp. 1096 - 1105
• Main Authors: Furtado, William Eduardo, Cardoso, Lucas, de Medeiros, Paula Brando, Lehmann, Nicollas Breda, de Aguiar Bertaglia, Elisabeth, da Costa Marchiori, Natalia, Bertoldi, Fabiano Cleber, Martins, Maurício Laterça
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01-12-2021; Springer Nature B.V
• Subjects: Antiparasitic agents; Biomass; Health Promotion and Disease Prevention; Infectious Diseases; Lernaea cyprinacea; Medicine; Medicine & Public Health; Oleoresins; Original; Original Article; Pinus; Phytotherapic; Acicule; Oleoresin; Anchor worm; Pinus
• ISSN: 0971-7196; 0975-0703
• DOI: 10.1007/s12639-021-01400-2

This study evaluated the potential of alternative treatments against larval stages of
Lernaea cyprinacea
. For in vitro test, the nanoemulsified oils of
Pinus
sp. acicule and oleoresin were evaluated, along with Biogermex® (commercial product based on citrus biomass). For this, the motility of five larvae of the same stage (nauplii or copepodite) were evaluated in a 96-well microplate. Using the best results, on the in vivo test, fries of
Rhamdia quelen
were submitted to a long-term immersion bath (96 h) containing different concentrations of the product diluted directly in the water. It was possible to notice the antiparasitic potential of the oleoresin and the acicule of
Pinus
sp., as well as the citrus biomass extract against the parasites. The nanoemulsified oils successfully inhibited the development of nauplii (10 mg L
−1
in 24 h) and the fries showed to be tolerant to the presence of the compound (LC
50
96 h—16.74 mg L
−1
). The concentration of 30.5 mg L
−1
of Biogermex® eliminated the copepodites within 24 h, being more efficient than
Pinus
sp. when tested at the same stage, at the times analyzed. The results obtained indicate a potential use of these compounds as prophylactic agents against
L. cyprinacea
.