Advances in Colorimetric Assay Based on AuNPs Modified by Proteins and Nucleic Acid Aptamers

This review is focused on the biosensing assay based on AuNPs (AuNPs) modified by proteins, peptides and nucleic acid aptamers. The unique physical properties of AuNPs allow their modification by proteins, peptides or nucleic acid aptamers by chemisorption as well as other methods including physical...

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Bibliographic Details
Published in:	Chemosensors Vol. 9; no. 10; p. 281
Main Authors:	Melikishvili, Sopio, Piovarci, Ivan, Hianik, Tibor
Format:	Journal Article
Language:	English
Published:	Basel MDPI AG 01-10-2021
Subjects:	Acids aggregation Antibiotics Aptamers Assaying AuNPs Avidin Biosensors Carbodiimide Chemical modification Chemisorption Chloride chymotrypsin Colorimetry Enzymes Ethanol Gold Immobilization Ligands Nanoparticles Nucleic acids Optical properties Peptides Physical properties Proteins Sodium Streptavidin trypsin β-casein
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Summary:	This review is focused on the biosensing assay based on AuNPs (AuNPs) modified by proteins, peptides and nucleic acid aptamers. The unique physical properties of AuNPs allow their modification by proteins, peptides or nucleic acid aptamers by chemisorption as well as other methods including physical adsorption and covalent immobilization using carbodiimide chemistry or based on strong binding of biotinylated receptors on neutravidin, streptavidin or avidin. The methods of AuNPs preparation, their chemical modification and application in several biosensing assays are presented with focus on application of nucleic acid aptamers for colorimetry assay for determination of antibiotics and bacteria in food samples.
ISSN:	2227-9040 2227-9040
DOI:	10.3390/chemosensors9100281