A high melting structure in DNA distinguishes phases of the cell cycle

A high melting structure in DNA distinguishes phases of the cell cycle

Differential scanning microcalorimetry of the nuclei of dividing CHO cells revealed DNA structures that showed structural transitions at 60, 76, 88, and 105 degrees C (transitions I to IV, respectively). In cultures synchronized by isoleucine deprivation the enthalpies of transitions I and II were r...

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Bibliographic Details
Published in:Experimental cell research Vol. 177; no. 1; p. 221
Main Authors: Rice, G A, Touchette, N A, Creager, A N, Goldberg, J, Cole, R D
Format: Journal Article
Language:English
Published: United States 01-07-1988
Subjects:
Animals
Calorimetry, Differential Scanning
Cell Cycle
Cricetinae
DNA - biosynthesis
Female
Hot Temperature
Interphase
Isoleucine - physiology
Kinetics
Mitosis
Ovary
Thermodynamics
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Summary:Differential scanning microcalorimetry of the nuclei of dividing CHO cells revealed DNA structures that showed structural transitions at 60, 76, 88, and 105 degrees C (transitions I to IV, respectively). In cultures synchronized by isoleucine deprivation the enthalpies of transitions I and II were rather constant throughout the cell cycle. While the sum of the enthalpies of III and IV was nearly constant, the ratio of IV to III varied substantially from one phase of the cycle to another. A high IV:III ratio of 6 characterized G1 while S phase gave a IV:III ratio of about 2. Cells containing metaphase chromosomes also showed a IV:III ratio near 2. The IV:III ratio for CHO cells showed a progressive decrease as the cells were maintained in isoleucine-free medium from 0 to 6 days.
ISSN:0014-4827
DOI:10.1016/0014-4827(88)90040-7