Regional cerebral blood volume reduction in transgenic mutant APP (V717F, K670N/M671L) mice

• Published in: Neuroscience letters Vol. 365; no. 3; pp. 223 - 227
• Main Authors: Wu, Ed X, Tang, Haiying, Asai, Tomohiro, Yan, Shi Du
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 29-07-2004; Elsevier
• Subjects: Alzheimer’s disease; Animals; Beta-amyloid; Biological and medical sciences; Blood Volume - genetics; Brain - anatomy & histology; Brain - blood supply; CBV; Cerebral blood volume; Contrast Media; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Fundamental and applied biological sciences. Psychology; Magnetic Resonance Imaging - methods; Male; Medical sciences; Mice; Mice, Mutant Strains; Mice, Transgenic; Neurology; Vascular; Vertebrates: nervous system and sense organs; Cerebral blood volume; AD; Vascular; Alzheimer’s disease; CBV; Beta-amyloid; Nervous system diseases; Alzheimer disease; Rodentia; Central nervous system; Transgenic animal; Cerebral disorder; Vertebrata; Mammalia; Mouse; Blood volume; β Amyloid protein; Central nervous system disease; Degenerative disease; Mutation; Alzheimer's disease
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2004.05.004

Recent advance in nuclear magnetic resonance (NMR) microimaging has enabled in vivo cerebral blood volume (CBV) mapping with high spatial resolution. Using an intravascular susceptibility contrast agent and T
2-weighted magnetic resonance imaging (MRI) on a 9.4
T NMR microimager, the regional CBV was measured in mice as the transverse relaxation increase induced by the contrast agent. CBV maps in an Alzheimer’s disease mouse model at resting state were obtained and examined. Four-month-old male transgenic mutant APP (V717F, K670N/M671L) mice (
N = 10) and littermate wild-type controls (
N = 12) were used. Regional analysis of the multi-slice CBV maps revealed statistically significant CBV reductions among the APP mice in cerebral cortex (−9.29%,
P = 0.0002), hippocampus (−4.22%,
P = 0.02), and thalamus (−5.21%,
P = 0.03), indicating an early change of microvasculature in these selected regions. No significant difference was found in olfactory bulb, pons, midbrain, superior colliculus, medulla, and cerebellum.