Properties and sidedness of 5'-nucleotidase in rat red cell ghosts

The occurrence of a Mg++-activated 5'-AMPase activity in rat ghosts is demonstrated. This activity is inhibited by the alpha, beta-methyleneadenosine 5'-diphosphate, a specific inhibitor of 5'-nucleotidase. The enzyme has an apparent Km of approximately 90 microM, and is located on th...

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Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 112; no. 2; p. 407
Main Authors:	Agnisola, C, Foti, L, Trara Genoino, I, Sciurba, M
Format:	Journal Article
Language:	English
Published:	United States 29-04-1983
Subjects:	5'-Nucleotidase Animals Chemical Phenomena Chemistry Enzyme Activation - drug effects Erythrocyte Membrane - enzymology Erythrocytes - enzymology Magnesium - pharmacology Nucleotidases - antagonists & inhibitors Nucleotidases - blood Nucleotidases - isolation & purification Rats Rats, Inbred Strains
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Summary:	The occurrence of a Mg++-activated 5'-AMPase activity in rat ghosts is demonstrated. This activity is inhibited by the alpha, beta-methyleneadenosine 5'-diphosphate, a specific inhibitor of 5'-nucleotidase. The enzyme has an apparent Km of approximately 90 microM, and is located on the exterior side of the plasma membrane.
ISSN:	0006-291X
DOI:	10.1016/0006-291X(83)91478-X